The MagBeads FastRNA Kit for Feces is a scalable and automatable magnetic bead-based RNA purification technology, designed for the efficient isolation of total RNA from fecal samples. Ready-to-use versions are available (Cat. No. 117040300 & Cat. No. 117040400).
The composition of fecal samples is largely dependent on diet, which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. These compounds impair sample homogenization, decreasing both the quantity and quality of the extracted RNA. The MagBeads FastRNA Kit for Feces integrates our 1) state-of-the-art beads-beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNase I Enzyme treatment allows the complete removal of DNA. The MagBeads FastRNA Kit for Feces streamlines RNA purification by overcoming the challenges associated with diverse fecal sample types, making the process both hassle-free and time efficient. A DNase I treatment step effectively removes contaminated DNA, ensuring high RNA yield and purity. The extracted RNA is immediately compatible with downstream applications such as RT-qPCR amplification, eliminating the need for additional inhibitor or DNA removal steps.
Performance
Figure 1. RNA Yield and Purity from Fecal Samples Extracted with MagBeads FastRNA Kit for Feces. RNA yield and purity (A260/A280 and A260/A230 ratios) obtained from three human fecal sources. Each dot represents an individual sample processed with the MagBeads FastRNA Kit for Feces.
Figure 2. Performance evaluation of RNA extracted from human fecal samples using the MagBeads FastRNA Kit for Feces (MP) compared to Competitor M. RNA was independently extracted from human fecal samples (two extractions per sample, each dot on the plot represents one extraction). The RNA yield, purity (A260/A230 ratio) and integrity were assessed using spectrophotometry and agarose gel electrophoresis.
Figure 3: Performance evaluation of RNA extracted from animal fecal samples using the MagBeads FastRNA Kit for Feces (MP) compared to Competitor M. RNA was independently extracted from animal fecal samples (two extractions per sample, each dot on the plot represents one extraction). The RNA yield, purity (A260/A230 ratio) and integrity were assessed using spectrophotometry and agarose gel electrophoresis. In addition, the extracted RNA was also assessed for its amplifiability using qPCR and the Ct values given by MP are earlier than Competitor M. The horizontal bars indicate the median value.
Application Notes
The MagBeads FastRNA Kit for Feces was designed for rapidly isolating RNA from Feces using Magnetic Beads automation technology.
Usage Statement
Research Use Only (RUO)
Key Applications
RT-PCR
| SKU | 116588050 |
| Application Notes | The MagBeads FastRNA Kit for Feces was designed for rapidly isolating RNA from Feces using Magnetic Beads automation technology. |
| Base Catalog Number | 6588050 |
| Product Line | MagBeads |
| Components | Lysing Matrix YB (5 x 2 mL), DNase I (53 μL), DNase I Buffer (1.4 mL), Magnetic Beads F (160 μL), Buffer PC (1.1 mL), Buffer MFR1 (2.8 mL), Buffer MFR2 (280 μL), Buffer MFR3 (3 mL), Buffer MFR4 (4.4 mL), Buffer MFR5 (4.4 mL), Buffer MFR6 (8.8 mL), RNase-Free Water (700 μL). |
| Format | Kit |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Feces |
| Storage | Store DNase I , Buffer MFR2, Magnetic Beads F, and Buffer PC at 2-8˚, the remaining kit contents should be stored at 15-25°C. |
| Usage Statement | Research Use Only (RUO) |
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