MagBeads FastRNA Kit for Blood (Ready-to-Use for MagFlex-96)

MagBeads FastRNA Kit for Blood (Ready-To-Use for MagFlex-96) is a scalable and automatable magnetic bead-based RNA purification technology that is designed for total isolation of RNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate). RNA degradation prevention is the backbone of RNA purification. The use of our specially formulated Buffer MBL containing huge amounts of chaotropic ions omits the need of excessive homogenization and enables highly efficient lysis with the immediate inactivation of RNases, which are typically present in most biological materials. The MagBeads FastRNA Kit for Blood simplifies the RNA purification process by eliminating the need for complicated erythrocyte lysis and isolation of leukocyte pellet, making it hassle-free and time efficient. Contaminated DNA is removed by a DNase I treatment step. Simplified dual washing steps largely remove salts, metabolites, and macromolecular cellular components. Pure RNA extracts of high yield and purity, compatible with downstream applications such as RT-qPCR amplification, are then eluted under low ionic strength conditions with RNase-free water.

Figure 1: High Quality RNA Purification. MagBeads FastRNA Kit for Blood offers high quality and highly consistent RNA extraction efficiency from blood of various anti-coagulants.

 

Application Notes

The MagBeads FastRNA Kit for Blood (Ready-To-Use for MagFlex-96™) is designed for magnetic bead-based purification of RNA from blood samples.

Usage Statement

Research Use Only (RUO)

Key Applications

PCR, qPCR amplification