MagBeads FastDNA Kit for Blood (Ready-to-Use for MagFlex-96)

The MagBeads FastDNA Kit for Blood offers an efficient solution for genomic DNA purification from various blood samples, including fresh and frozen human whole blood preserved in common anticoagulants, buffy coat, and saliva. Designed to be compatible with the Magflex-96 System, it supports high-throughput processing of up to 96 samples and accommodates varying sample volumes from 50 µL to 500 µL. The kit features a rapid, integrated approach for DNA isolation by combining lysis and binding steps, omitting the need for additional sample processing steps. The Magnetic Beads used in MagBeads FastDNA Kit for Blood simplifies the DNA purification process by eliminating complicated RNA removal step, ensuring highly efficient and specific binding of DNA. Specialized wash buffers included in the kit deliver high washing efficiency, removing proteins, salts, and other impurities through a quick, streamlined wash procedure. The DNA eluted in ionic strength elution buffer is of high quality, free from contaminants, and fully compatible with downstream applications such as PCR and other molecular techniques.

Figure 1: Performance of MagBeads FastDNA Kit for Blood with varying blood volumes from different individuals. The MagBeads FastDNA Kit for Blood demonstrated good reproducibility across wells. Wells 1–12: 400 µL EDTA blood; Wells 13–24: 500 µL EDTA blood. Improved DNA yield was observed with higher input volume.

Figure 2: Extraction Efficiency of MagBeads FastDNA Kit for Blood Using MagFlex-96™ System. The MagBeads FastDNA Kit for Blood showed high-quality and consistent gDNA extraction, with high yield and desirable purity ratios across blood preserved in different anticoagulants and buffy coat. 1-3: 200 µL EDTA blood, 4-6: 200 µL sodium citrate blood; 7-9: 200 µL heparin blood; 10-12: 500 µL EDTA blood, 13-15: 100 µL buffy coat.

Figure 3: (A) gDNA was extracted from blood samples under different conditions and analyzed on a 1% agarose gel. Lanes are labeled as follows: "Short" (~1106 bp), "Medium" (~1424 bp), "Long" (~5778 bp), and "NTC" (Negative Template Control, no visible band). (B) Long fragments of the human BRCA gene were amplified via Endpoint PCR. DNA extracted with the MagBeads FastDNA Kit (MP) successfully produced the long fragment gene product, while DNA extracted with the Competitor T kit failed to amplify the long fragment. 

Figure 4: DNA Integrity Analysis of Blood gDNA Extracted with MP Biomedicals MagBeads FastDNA Kit for Blood. Genomic DNA was extracted from 200 µL blood using the MagBeads FastDNA Kit for Blood and compared to other competitor kits. The MagBeads FastDNA Kit for Blood yielded DNA with superior integrity and higher molecular weight, as assessed by the Agilent Technologies 4150 TapeStation Genomic DNA ScreenTape system.

Application Notes

The MagBeads FastDNA Kit for Blood (Ready-To-Use for MagFlex-96™) is designed for magnetic bead-based purification of DNA from blood samples.

Usage Statement

Research Use Only (RUO)

Key Applications

PCR, qPCR amplification