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Normal goat serum is commonly used for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies. It can successfully reduce nonspecific binding of proteins and antibodies to reaction surfaces and non-specific binding sites. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk.
Application Notes
Whole serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Whole sera are also useful as controls for testing general and specific antibody purification methods.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays, Blocking, Immunohistochemistry, Immunocytochemistry, Double Immunofluorescence, Enzyme Immunoassays (EIAs)
| SKU | 0855984 |
| Alternate Names | Negative Control; Control |
| Application Notes | Whole serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Whole sera are also useful as controls for testing general and specific antibody purification methods. |
| Base Catalog Number | 55984 |
| Concentration | 58.1 mg/ml |
| Format | lyophilized |
| Formulation Details | Lyophilized | Delipidated; Lyophilized from PBS with azide. |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | The serum is delipidated, dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |