Protease, from Staphylococcus aureus V8 strain, ~500 U/mg

Name: Protease, from Staphylococcus aureus V8 strain, ~500 U/mg
SKU: 02151972-CF

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SKU: 02151972-CF

Key features and details

Used for selective cleavage of proteins for amino acid sequence determination or peptide mapping
Cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues

Synonyms

Endoproteinase Glu-C

CAS Number:

66676-43-5

Molecular Weight:

27,700 (sedimentation equilibrium)

MDL Number:

MFCD00132092

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Product Description

Protease

Application Notes

Protease V8 is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping. The Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Peptide Mapping

Specifications

SKU	02151972-CF
Alternate Names	Endoproteinase Glu-C
Applications	Peptide Mapping
Application Notes	Protease V8 is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping. The Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues.
Base Catalog Number	151972
CAS #	66676-43-5
Extinction Coefficient	E1%280 = 4.26
Format	Powder
Hazard Statements	H315-H319-H334-H335
Molecular Weight	27,700 (sedimentation equilibrium)
Preparation Method	The enzyme was isolated, by the method of Drapeau et al., from the culture filtrate of S. aureus V8 strain. The preparation is homogenous on ultracentrifugation and disc electrophoresis.
Safety Symbol	GHS07, GHS08
Solubility	Soluble in aqueous buffers (such as 10 mM Tris, pH 7.8 at 1 mg/mL)
Source	Staphylococcus aureus V8 strain
Specific Activity	>500 u/mg
Storage	-20 °C
Unit Definition	One unit will hydrolyze casein to change A280 0.001 per minute, pH 7.8, at 37 °C.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.