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Hyaluronidase, >1500 U/mg
SKU: 02151274-CF
Hyaluronidase
Application Notes
Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated. Hyaluronidase is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Drug dispersion & delivery
| SKU | 02151274-CF |
| Alternate Names | Hyaluronate 4-glycanohydrolase; hyaluronate lyase; hyaluronoglucosaminidase; 3.2.1.35 |
| Application Notes | Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated. Hyaluronidase is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible. |
| Base Catalog Number | 151274 |
| Biochemical Physiological Actions | Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates. |
| CAS # | 37326-33-3 |
| EC Number | 253-464-3 |
| Extinction Coefficient | ~8 |
| Format | lyophilized, Salt free |
| Hazard Statements | H317 |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| Source | Ovine Testes |
| Specific Activity | >1500 u/mg material |
| Specificity | Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B); primarily to tetrasaccharide residues. Monosaccharides are not liberated. |
| Unit Definition | Amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37°C and pH 4.0 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |