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DL-Dithiothreitol, molecular biology reagent
SKU: 02194821-CF
DL-Dithiothreitol, molecular biology reagent
Application Notes
DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Reducing Agent
| SKU | 02194821-CF |
| Alternate Names | 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent racemic; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent |
| Application Notes | DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. |
| Base Catalog Number | 194821 |
| Beilstein Registry Number | 1719757 |
| CAS # | 3483-12-3 |
| EC Number | 248-531-9 |
| Format | Powder |
| Grade | Molecular Biology Grade |
| Half Life | .1 M KPO4 buffer (Half Life in hours):- pH 6.5 at 2 °C: 4, pH 7.5 at 2 °C: 1, pH 8.5 at 2 °C: 1.4, pH 8.5 at °C: 11, pH 8.5 at 4 °C: .2, pH 8.5 at 2 °C with addition of .1 mM Cu2+: .6, pH 8.5 at 2 °C with addition of 1. mM EDTA: 4 |
| Hazard Statements | H302-H315-H319-H335 |
| Melting Point | 40 - 50°C |
| Molecular Formula | C4H10O2S2 |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| Purity | ≥99% |
| RTECS Number | EK1610000 |
| Safety Symbol | GHS07 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |