Hyaluronidase from bovine testes, ≥300 U/mg

Name: Hyaluronidase from bovine testes, ≥300 U/mg
SKU: 02100740-CF

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SKU: 02100740-CF

Synonyms

Hyaluronate Glycanohydrolase; Hyaluronate lyase; chondroitinase; :Hyaluronoglucosaminidase Hyaluronate 4-glycanohydrolase

CAS Number:

37326-33-3

EC Number:

253-464-3

MDL Number:

MFCD00131351

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Product Description

Hyaluronidase

Application Notes

Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated. Hyaluronidase is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Drug dispersion & delivery

Specifications

SKU	02100740-CF
Alternate Names	Hyaluronate Glycanohydrolase; Hyaluronate lyase; chondroitinase; :Hyaluronoglucosaminidase Hyaluronate 4-glycanohydrolase
Application Notes	Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated. Hyaluronidase is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.
Base Catalog Number	100740
Biochemical Physiological Actions	Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
CAS #	37326-33-3
EC Number	253-464-3
Extinction Coefficient	~8 (lit.)
Format	Lyophilized powder form
Hazard Statements	H317
Personal Protective Equipment	Eyeshields, Gloves, Respirator filter
Source	Bovine Testes
Specific Activity	≥300 U/mg
Specificity	Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B); primarily to tetrasaccharide residues. Monosaccharides are not liberated
Unit Definition	Amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37°C and pH 4.0
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.