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Anti-rabbit IgG Fc affinity-purified goat antibody, peroxidase-conjugated, 2 mL
SKU: 0855690
Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG Fc and buffer salts.
Application Notes
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
| SKU | 0855690 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.) |
| Base Catalog Number | 55690 |
| Concentration | Total Protein: 0.6 mg/ml Titer: 1:23;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Purified |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rabbit |
| Specificity | It shows reactivity to rabbit Fc and IgG; cross-reactivity to other species may exist. Antibody activity to F(ab')<sub>2</sub>; IgA; IgM; or non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | SUGGESTED WORKING DILUTIONS: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 -1: 4,000 for Elisa |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |