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Anti-rabbit IgG (whole molecule) affinity-purified goat antibody, fluorescein-conjugated, 2 mg
In stock
SKU: 0855662
Product Description
Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.
Application Notes
Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Specifications
| SKU | 0855662 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). |
| Base Catalog Number | 55662 |
| Concentration | Total Protein: 1.0 mg/mL; Active Antibody: 1.0 mg/mL |
| Conjugate | FITC |
| Format | lyophilized |
| Formulation Details | Purified |
| Grade | Molecular Grade |
| Host | Goat |
| Immunogen | Purified rabbit IgG |
| Pack Size | 2 mg |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rabbit |
| Specificity | Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | Anti-Rabbit IgG (whole molecule)-FITC antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:160. A minimum working dilution of 1:160 may be used on formalin-fixed, paraffin-embedded human tonsil sections and Anti-Human IgG as the primary antibody. This antibody was used for immunefluorescence of Alfalfa root and leaf sections stained with anti-Medicago sativa GSK-3-like kinase primary antibody. For immunostaining of human testis cryosections, a dilution of 1:200 was used with N-cadherin primary antibody. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
![MP COA [55662] lot ID: M8210](https://www.mpbio.com/static/version1781469166/frontend/Mpbio/luma/en_US/images/document.png "MP COA [55662] lot ID: M8210")
Datasheet
Datasheet
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