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Isoamyl alcohol
SKU: 02194059-CF
Isoamyl Alcohol
Application Notes
Isoamylalcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
DNA purification | Conjugation
| SKU | 02194059-CF |
| Alternate Names | Isopentyl alcohol; 3-Methyl-1-butanol; 3-Methylbutanol; Isopentyl alcohol; Isobutyl carbinol; Primary isoamyl alcohol; Fermentation amyl alcohol |
| Application Notes | Isoamylalcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process. |
| Auto Ignition | 662° F (USCG, 1999) |
| Base Catalog Number | 194059 |
| Beilstein Registry Number | 1718835 |
| Boiling Point | 270° F at 760 mm Hg (USCG, 1999) |
| CAS # | 123-51-3 |
| Density | 0.81 at 68° F (USCG, 1999) |
| EC Number | 204-633-5 |
| Flash Point | 114° F (USCG, 1999) |
| Format | Liquid |
| Grade | ACS grade |
| Hazard Statements | H226-H332-H335 |
| Melting Point | -179° F (NIOSH, 2016) |
| Molecular Formula | C5H12O |
| Molecular Weight | 88.15 g/mol |
| Personal Protective Equipment | Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge , respirator filter |
| Purity | ≥98.5% |
| Refractive Index | n20/D 1.406(lit.) |
| RTECS Number | EL5425000 |
| Safety Symbol | GHS02, GHS07 |
| Solubility | 2 % at 57° F (NIOSH, 2016) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Density | 3.04 (AIR=1) |
| Vapor Pressure | 28 mm Hg (NIOSH, 2016) |