Kit for general PCR applications, includes Taq DNA Pol at 5 U/µL, dNTPs at 10 mM each, 10x Incubation Buffer with and without MgCl2, MgCl2 at 25 mM
Application Notes
Taq DNA Polymerase is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. Other application include RT-PCR, DNA sequencing and DNA labeling.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Routine PCR
SKU | 11EPTQK101-CF |
Alternate Names | Taq polymerase |Routine PCR Enzyme |Taq Enzyme with Nucleotides Mix |
Application Notes | Taq DNA Polymerase is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. Other application include RT-PCR, DNA sequencing and DNA labeling. |
Biochemical Physiological Actions | Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities. |
Components | Taq DNA Pol at 5 U/µL |dNTPs at 10 mM each |10x Incubation Buffer with MgCl2|10x Incubation Buffer without MgCl2 |MgCl2 at 25 mM |
Concentration | 5U/μL |
Format | Kit |
Grade | Molecular Biology Grade |
Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
Protein or Enzyme Type | Molecular Biology Enzymes |
Unit Definition | One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |