Sterile 80% Glycerol solution is less viscous and more convenient to pipette than 100% glycerol.
Application Notes
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It has been used in purification of bacteriophage λ particles by step gradients, calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNA, DEAE-dextranmediated transfection of DNA into cells, DNA sequencing reactions, and mediation of mispriming in PCR. The use of glycerol in improved focusing of alkaline pH protein samples in immobilized pH gradient (IPG) strips has been published. Studies of the effect of glycerol on separation of DNA fragments by capillary electrophoresis have been reported.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
| SKU | 113055034-CF |
| Alternate Names | 1,2,3-Propanetriol; Glycerin |
| Application Notes | Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. It has been used in purification of bacteriophage λ particles by step gradients, calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNA, DEAE-dextranmediated transfection of DNA into cells, DNA sequencing reactions, and mediation of mispriming in PCR. The use of glycerol in improved focusing of alkaline pH protein samples in immobilized pH gradient (IPG) strips has been published. Studies of the effect of glycerol on separation of DNA fragments by capillary electrophoresis have been reported. |
| Auto Ignition | 698 °F (Lit.) |
| Base Catalog Number | 3055034 |
| Beilstein Registry Number | 635685 |
| Boiling Point | 182 °C/20 mmHg (Lit.) |
| CAS # | GLYCEROL 56-81-5; WATER 7732-18-5 |
| Concentration | 0.8 |
| Density | 1.25 g/mL (Lit.) |
| EC Number | 200-289-5 |
| Flash Point | 160 °C/320 °F (Lit.) |
| Format | Solution |
| Formulation Details | 800 mL of 100% ACS grade Glycerol is combined with 200 mL of H2O and mixed well. It is then aliquoted into P.C. bottles and autoclaved. |
| Molecular Formula | HOCH2CH(OH)CH2OH |
| Molecular Weight | 92.09 |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| pH | 6.0-7.0 (10% solution) |
| Refractive Index | n20/D 1.474 |
| Solubility | Miscible in water. |
| Sterility | Sterile |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | <1 mmHg (20 °C) |
![MP SDS [3055034]](https://www.mpbio.com/static/version1715349838/frontend/Mpbio/default/en_US/images/document.png "MP SDS [3055034]")