Tween® 80, cell culture reagent

Tween® 80, cell culture reagent

Synonyms Polyoxyethylenesorbitan monooleate ;POE (20) sorbitan monooleate; Polyethylene glycol sorbitan monooleate ;Polysorbate 80

CAS Number: 9005-65-6 Molecular Weight: 604.822 g/mol

MDL Number: MFCD00082107

SKU 02194725-CF
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Description

Product Description

Tween® 80

Application Notes

Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli , and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Selective protein extraction | Isolation of nuclei from mammalian cell lines

Specifications
SKU 02194725-CF
Alternate Names Polyoxyethylenesorbitan monooleate ;POE (20) sorbitan monooleate; Polyethylene glycol sorbitan monooleate ;Polysorbate 80
Application Notes Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli , and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Base Catalog Number 194725
CAS # 9005-65-6
Critical Micelle Concentration 0.012 mM (13-15 mg/L)
Format Liquid
Grade Cell Culture Grade
Hazard Statements H319
Molecular Weight 604.822 g/mol
Personal Protective Equipment Eyeshields, Gloves
RTECS Number WG2932500
Safety Symbol GHS07
Specific Gravity 1.06 - 1.09
Sterilization of Solutions Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.