Cells often serve as a starting point to understand and identify the basic biochemical and physical processes of life, including cell health, proliferation, differentiation, function and death. Cell analysis enables investigation of basic cell biology, disease pathology and drug discovery. MP Bio provides comprehensive solutions with high sensitivity and low cytotoxicity for cellular analysis, including cell viability determination, mitophagy detection in mammalian cells and cellular senescence detection.
Trypan blue, 0.4% solution in PBS, 100 mL
Trypan Blue is a blue acid dye with a strong affinity for cellulose. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
1X Trypsin-EDTA solution (0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA), 100 mL
Used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.0
10X Trypsin HBSS w/o calcium and magnesium, 100 mL
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.