Affinity Purified Rabbit Anti-Mouse IgG (H&L), 2 mg
SKU: 0855480
Product is rabbit affinity purified antibody to mouse IgG (whole molecule) and buffer salts.
Application Notes
Rabbit Affinity Purified Antibody to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Precipitating antibody |Immunoprecipitation
| SKU | 0855480 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Rabbit Affinity Purified Antibody to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55480 |
| Concentration | Total Protein: 1.1 mg/mL; Active Antibody: 1.0 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Liquid |
| Grade | Molecular Grade |
| Host | Rabbit |
| Immunogen | Mouse IgG |
| Isotype | IgG |
| Pack Size | 2 mg |
| Preparation Method | The affinity purified antibody is prepared from the specific rabbit IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | It shows reactivity to mouse IgG; cross-reactivity to other species may exist. |
| Typical Working Concentration | Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for ELISA |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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