Product is the lyophilized powder of rabbit IgG fraction to human albumin and buffer salts.
Application Notes
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
| SKU | 0855029 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. |
| Base Catalog Number | 55029 |
| Concentration | Total Protein: 15.1 mg/mL; Antibody Protein: 5 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Rabbit |
| Immunogen | Human albumin |
| Pack Size | 2 mL |
| Preparation Method | The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human |
| Specificity | It shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present. |
| Sterility | Filtered through a .22 µm filter |
| Typical Working Concentration | Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA/FIA |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [0855029] lot ID: U1123149710](https://www.mpbio.com/static/version1742546773/frontend/Mpbio/default/en_US/images/document.png "MP COA [0855029] lot ID: U1123149710")
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