2-Mercaptoethanol
Application Notes
2-Merceptoethanol is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Solubilization of proteins
| SKU | 0219024283 |
| Alternate Names | β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol |
| Application Notes | 2-Merceptoethanol is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. |
| Auto Ignition | 295°C |
| Base Catalog Number | 19024283 |
| Beilstein Registry Number | 773648 |
| Boiling Point | 315 to 316° F at 742 mm Hg (decomposes) (NTP, 1992) |
| CAS # | 60-24-2 |
| Density | 1.1143 at 68° F (NTP, 1992) |
| EC Number | 200-464-6 |
| Flash Point | 165° F (NFPA, 2010) |
| Format | Liquid |
| Grade | Standard Grade |
| Hazard Statements | H301-H310 + H330-H315-H317-H318-H410 |
| Melting Point | -148° F (NTP, 1992) |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.129 g/mol |
| Pack Size | 250 mL |
| Personal Protective Equipment | Faceshields, full-face respirator , Gloves, Goggles, respirator filter |
| Purity | ≥98% |
| Refractive Index | n20/D 1.500(lit.) |
| RTECS Number | KL5600000 |
| Safety Symbol | GHS05, GHS06, GHS09 |
| Solubility | greater than or equal to 100 mg/mL at 68° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Density | 2.69 (NTP, 1992) (Relative to Air) |
| Vapor Pressure | 1 mm Hg at 68° F (NTP, 1992) |
![MP COA [190242] lot ID: MR29305](https://www.mpbio.com/static/version1744790984/frontend/Mpbio/default/en_US/images/document.png "MP COA [190242] lot ID: MR29305")