Product is the lyophilized powder of rat IgG and buffer salts.
Application Notes
Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG. It is suitable for use as a positive control in antibody identification screening techniques.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis
| SKU | 08559512-CF |
| Alternate Names | IgG from rat serum; Rat IgG |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG. It is suitable for use as a positive control in antibody identification screening techniques. |
| Base Catalog Number | 55951 |
| Concentration | Total Protein: 10.4 mg/mL; Volume: 2 mL |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized |
| Host | Rat |
| Isotype | IgG |
| Pack Size | 1 Box |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Preparation Method | Rat IgG is purified from pooled rat serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, and filtered through a 0.22 µm filter, vialed and lyophilized. No preservative is added. |
| Purity | > 99%; lyophilized |
| Solubility | Soluble in water |
| Source | Rat |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Powered by Bioz