3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide
Application Notes
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Histochemical/Cytochemical Reagent
| SKU | 02102227-CF |
| Alternate Names | MTT; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide |
| Application Notes | Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems. |
| Base Catalog Number | 102227 |
| Beilstein Registry Number | 4081397 |
| CAS # | 298-93-1 |
| EC Number | 206-069-5 |
| Hazard Statements | H315-H319-H335-H341 |
| Melting Point | 195 deg C (decomposes) |
| Molecular Formula | C18H16BrN5S |
| Molecular Weight | 414.325 g/mol |
| Personal Protective Equipment | Eyeshields, Faceshields, Gloves, respirator cartridges |
| Purity | ~98% |
| RTECS Number | XF8060000 |
| Safety Symbol | GHS07,GHS08 |
| Solubility | Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a>, 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 20 mg/mL |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | 376 ± 2 nm; 242 ± 2 nm |
| Vapor Pressure | 5.11X10-11 mm Hg at 25 deg C /Estimated/ |
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