Product is the lyophilized powder of goat F(ab')2 fragment to mouse complement C3 and buffer salts.
Application Notes
Goat F(ab')2 Fragment to Mouse Complement C3 is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Precipitating antibody |Immunoprecipitation
| SKU | 0855474 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Goat F(ab')2 Fragment to Mouse Complement C3 is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). |
| Base Catalog Number | 55474 |
| Concentration | Total Protein: 6.5 mg/mL; Antibody Protein: 4 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Immunogen | Full length protein (Mouse) |
| Pack Size | 2 mL |
| Preparation Method | The F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, 0.05% sodium azide, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | It shows reactivity to mouse C3; cross-reactivity to other species may exist. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
