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Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide

NZ$67.41
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates.

SKU: 02150177-CF

CAS Number:
25988-63-0
Molecular Weight:
402.54 g/mol
MDL Number:
MFCD00237230
Product Description

Poly-L-Lysine Hydrobromide

Application Notes

Poly-L-Lysine is typically used as a coating substrate for culture dishes, slides, etc. It can be used in conjugation to methotrexate for increased drug transport. For Microencapsulation of islets. It is also use in simple reproducible procedure for chromosomal preparations from a variety of tissues. For immobilation of living cells in biocompatible semipermeable microcapsules. In the preparation of polycationic beads. Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. Poly-lysine with mol. wt. >70,000 is useful in promoting cell adhesion to solid substrates.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Coating substrate for culture dishes; slides | Cell adhesion | Vector for gene therapy

Specifications
SKU 02150177-CF
Application Notes Poly-L-Lysine is typically used as a coating substrate for culture dishes, slides, etc. It can be used in conjugation to methotrexate for increased drug transport. For Microencapsulation of islets. It is also use in simple reproducible procedure for chromosomal preparations from a variety of tissues. For immobilation of living cells in biocompatible semipermeable microcapsules. In the preparation of polycationic beads. Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. Poly-lysine with mol. wt. >70,000 is useful in promoting cell adhesion to solid substrates.
Base Catalog Number 150177
Biochemical Physiological Actions Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
CAS # 25988-63-0
Format Powder
Molecular Weight 402.54 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Typical Working Concentration Stock Solution for coating of culture dishes/slides: Dissolve 10 mg poly-L-lysine in 1 mL sterile, deionized water. This creates a 1% solution. Effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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