FastDNA™ SPIN Kit for Soil
SKU: 116560200-CF
FastDNA™ SPIN Kit for Soil
Genomic DNA Extraction from Soil and Environmental Samples
The FastDNA™ SPIN Kit for Soil from MP Biomedicals is designed for genomic DNA extraction from complex soil and environmental samples. The kit combines mechanical bead beating using Lysing Matrix E tubes with chemical processing and silica-based spin-filter purification to support DNA recovery from mixed microbial communities and other biological materials found in environmental matrices.
This integrated workflow helps researchers prepare genomic DNA for downstream molecular biology applications, including PCR-based analysis, microbial detection, and sequencing workflows where sample quality and assay requirements have been validated.
How the Workflow Works
Soil samples are mechanically disrupted using a compatible FastPrep instrument and the supplied Lysing Matrix E tubes. Subsequent precipitation, DNA binding, washing, and elution steps help separate nucleic acids from cellular debris and environmental contaminants, including PCR-inhibitory compounds.
Processing parameters and reagent preparation should follow the current kit instructions and compatible instrument operating guidelines.
Research Applications
- Soil Microbiology: Extraction of genomic DNA for microbial community analysis and environmental microbiology research.
- Environmental DNA Research: DNA extraction from soil, sediment, and related environmental sample matrices.
- PCR-Based Molecular Analysis: Preparation of genomic DNA for microbial detection, amplification, and molecular research.
- Sequencing Workflows: Sample preparation for downstream sequencing applications where DNA quality and assay requirements have been validated.
Technical Information
Standard 2 mL Kit – 50 Preparations
| Product Name | FastDNA™ SPIN Kit for Soil |
| Catalog Number | 6560200 |
| SG Product SKU | 116560200-CF |
| Kit Format | 2 mL Lysing Matrix E tubes with silica SPIN filters |
| Pack Size | 50 preparations |
| Sample Input | Up to 500 mg of soil per preparation |
| Extraction Method | Mechanical bead beating, chemical processing, and silica-based spin-filter purification |
| Compatible Equipment | Compatible FastPrep instrument and additional laboratory equipment as specified in the user manual |
Kit Components
The standard 50-preparation kit includes:
- 50 × Lysing Matrix E tubes
- Sodium Phosphate Buffer
- MT Buffer
- PPS Solution
- Binding Matrix
- 50 × SPIN Modules
- 50 × Catch Tubes
- Concentrated SEWS-M
- DES
- BBS Gel Loading Dye
A compatible FastPrep instrument and additional common laboratory equipment are required. Refer to the current product user manual for complete instructions, reagent preparation, and equipment requirements.
Application Notes
Cited in over 15,000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
| SKU | 116560200-CF |
| Alternate Names | FastDNA Soil Kit |
| Applications | Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples. |
| Application Notes | Cited in over 15,000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors |
| Base Catalog Number | 6560200 |
| Product Line | FastDNA™ |
| Components | 50 x 2.0 mL tubes of Lysing Matrix E, 60 mL Sodium Phosphate Buffer, 8 mL MT Buffer, 25 mL of PPS Solution, 66 mL Binding Matrix, 50 Spin Modules, 50 Catch Tubes, 12 mL Concentrated SEWS-M, 20 mL DES, 200 µl BBS Gel Loading Dye |
| FastPrep Models | ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL) |
| Format | Kit |
| Hardness | Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515 |
| Personal Protective Equipment | If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (Labcoat and long-sleeves), and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices. |
| pH | Reagent: SEWS-M pH Range: 6.4-6.8 |
| Sample Type | Seeds, Spores, Bacteria (Gram +/-), Animal Tissues, Feces, Food, Fungi, Plants, Roots, Soil, Wastewater, Yeast |
| Sample Volume | up to 500 mg per prep |
| Specific Gravity | Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
1. What is the FastDNA SPIN Kit for Soil used for?
The FastDNA SPIN Kit for Soil is used to extract genomic DNA from soil and other environmental samples, including mixed microbial populations. The workflow combines FastPrep bead-beating lysis with silica-based DNA purification for downstream molecular research.
2. How much soil can be processed per extraction?
The standard 2 mL FastDNA SPIN Kit for Soil workflow accepts up to 500 mg of soil per preparation. Follow the current manual when working with unusual or highly inhibitory samples.
3. Does the kit include Lysing Matrix tubes?
Yes. The standard 50-preparation kit includes 50 Lysing Matrix E tubes (2 mL) for mechanical disruption using a compatible FastPrep instrument.
4. Is a FastPrep instrument required?
The validated workflow is designed for mechanical homogenization with a FastPrep instrument and Lysing Matrix E tubes. Confirm the supported instrument and adapter configuration before use.
5. How does the kit address PCR inhibitors in soil?
Following lysis, the protocol uses precipitation, silica-based DNA binding, and wash steps to help remove humic acids and other contaminants that can interfere with PCR. Removal efficiency can vary with the sample matrix.
6. Which environmental samples can be used?
The kit is intended for soil and other environmental materials such as sediments, sludge, compost, and rhizosphere-associated samples. Sample-dependent optimization may be needed.
7. Is the extracted DNA suitable for PCR?
The kit is designed to yield DNA suitable for PCR and other downstream molecular applications. Suitability for a particular assay or sequencing workflow should be evaluated using the resulting DNA quality and relevant controls.
8. What is the difference between FastDNA SPIN Kit for Soil and SPINeasy DNA Pro Kit for Soil?
Both are MP Biomedicals kits for soil DNA extraction. FastDNA SPIN Kit for Soil uses Lysing Matrix E and a silica-based spin-filter workflow, whereas SPINeasy DNA Pro Kit for Soil uses its own kit-specific reagents and Lysing Matrix YB. Select based on sample type, workflow, and downstream requirements.
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