SPINeasy® DNA Pro Kit for Soil, 50 Preps
SKU: 116546050
The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types, including those with low biomass or that are highly contaminated. Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components, which often prove to be challenging for downstream molecular analysis.
The SPINeasy DNA Pro Kit for Soil effectively lyses microbial populations, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA™ SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Performance Data
The SPINeasy DNA Pro Kit for Soil has been developed to accommodate a wide range of soil samples. The kit provides optimal yields, A260/A280, and A260/A230 ratios of DNA using fresh or frozen samples. It displays an exceptional ability to extract DNA from low biomass samples compared to competitor kits (Figure 1, DNA quality).
Figure 1. Soil samples with various biomass/ contaminant content (250 mg each) were processed with SPINeasy DNA Pro Kit for Soil and compared to a competitor kit. The DNA yield and purity (A260/A280and A260/A230 ratio) were assessed using a spectrophotometer. Each point of the plot represents a single extraction. The horizontal bars indicate the median value. DNA integrity was assessed using a 1% agarose gel. For low biomass samples, a similar proportion of DNA eluates were loaded to compare the performance of SPINeasy DNA Pro Kit for Soil and competitor kit (Q pro).
Application Notes
The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Usage Statement
Research Use Only (RUO).
Key Applications
Genomic, Molecular Biology, Nucleic Acid Isolation & Purification, PCR, qPCR, NGS, Shotgun sequencing, Microbiome analysis
| SKU | 116546050 |
| Alternate Names | SPINeasy Pro Soil Kit, DNA Extraction Kit for Soil |
| Application Notes | The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps. |
| Base Catalog Number | 65460 |
| Product Line | SPINeasy |
| Components | 50x Lysing Matrix YB, 2 mL RNase A Solution, 55 mL Buffer S1, 22 mL Buffer S2, 50 mL Buffer S3, 28 mL Buffer S4, 40 mL Buffer S5, 6 mL Buffer S6, 50x Column S, 50x 2 mL collection tubes, 50x 1.5 mL collection tubes |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Soil, Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil |
| Sample Volume | Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil |
| Storage | Store at Room Temperature (15-25℃) in a dry environment |
| Usage Statement | Research Use Only (RUO). |
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