SPINeasy® DNA Pro Kit for Soil, 50 Preps
SKU: 116546050
The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types, including those with low biomass or that are highly contaminated. Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components, which often prove to be challenging for downstream molecular analysis.
The SPINeasy DNA Pro Kit for Soil effectively lyses microbial populations, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA™ SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Performance Data
The SPINeasy DNA Pro Kit for Soil has been developed to accommodate a wide range of soil samples. The kit provides optimal yields, A260/A280, and A260/A230 ratios of DNA using fresh or frozen samples. It displays an exceptional ability to extract DNA from low biomass samples compared to competitor kits (Figure 1, DNA quality).
Figure 1. Soil samples with various biomass/ contaminant content (250 mg each) were processed with SPINeasy DNA Pro Kit for Soil and compared to a competitor kit. The DNA yield and purity (A260/A280and A260/A230 ratio) were assessed using a spectrophotometer. Each point of the plot represents a single extraction. The horizontal bars indicate the median value. DNA integrity was assessed using a 1% agarose gel. For low biomass samples, a similar proportion of DNA eluates were loaded to compare the performance of SPINeasy DNA Pro Kit for Soil and competitor kit (Q pro).
Application Notes
The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Usage Statement
Research Use Only (RUO).
Key Applications
Genomic, Molecular Biology, Nucleic Acid Isolation & Purification, PCR, qPCR, NGS, Shotgun sequencing, Microbiome analysis
| SKU | 116546050 |
| Alternate Names | SPINeasy Pro Soil Kit, DNA Extraction Kit for Soil |
| Applications | Genomic, Molecular Biology, Nucleic Acid Isolation & Purification, PCR, qPCR, NGS, Shotgun sequencing, Microbiome analysis |
| Application Notes | The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps. |
| Base Catalog Number | 65460 |
| Product Line | SPINeasy |
| Components | 50x Lysing Matrix YB, 2 mL RNase A Solution, 55 mL Buffer S1, 22 mL Buffer S2, 50 mL Buffer S3, 28 mL Buffer S4, 40 mL Buffer S5, 6 mL Buffer S6, 50x Column S, 50x 2 mL collection tubes, 50x 1.5 mL collection tubes |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Soil, Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil |
| Sample Volume | Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil |
| Storage | Store at Room Temperature (15-25℃) in a dry environment |
| Usage Statement | Research Use Only (RUO). |
1. What is the SPINeasy DNA Pro Kit for Soil used for?
The SPINeasy DNA Pro Kit for Soil is designed for the isolation and purification of microbial genomic DNA from soil and sediment samples, including challenging, inhibitor-rich, and low-biomass materials. The purified DNA can be used in downstream molecular biology research applications.
2. How much soil can be processed per preparation?
The kit supports 100–500 mg of soil per preparation. The optimal sample input depends on soil characteristics and should follow the current instruction manual.
3. Can the kit remove humic acid and other PCR inhibitors?
The kit includes an inhibitor-removal step designed to reduce soil-derived contaminants, including humic substances, that may interfere with PCR and other downstream molecular analyses. Removal efficiency may vary depending on the sample characteristics.
4. Is the extracted DNA suitable for 16S sequencing and metagenomics?
Yes. The extracted DNA is intended for downstream applications including 16S rRNA gene sequencing, whole-genome sequencing, PCR, and qPCR. Suitability for metagenomic and other sequencing workflows depends on DNA quality and the requirements of the downstream application.
5. What soil types can be used with the kit?
MP Biomedicals product information lists sand, clay, sediment, alkaline soil, and topsoil among the supported sample types. Extraction performance depends on sample biomass and inhibitor content. Follow the current protocol for recommended processing conditions.
6. Does the kit include bead-beating tubes?
Yes. The 50-preparation kit includes Lysing Matrix YB tubes for mechanical sample disruption, along with extraction reagents and spin-column components required for the DNA purification workflow.
7. Can I use a FastPrep instrument with this kit?
Yes. The protocol describes sample homogenization using a compatible FastPrep instrument and the supplied Lysing Matrix YB tubes. Refer to the current instruction manual for recommended instrument settings and equipment compatibility.
8. How does the SPINeasy DNA Pro Kit for Soil compare with the FastDNA SPIN Kit for Soil?
Both kits are designed to isolate DNA from soil samples using mechanical lysis and silica-based purification. The SPINeasy DNA Pro Kit for Soil uses Lysing Matrix YB tubes and features a workflow designed to improve DNA purity and reduce processing time while providing comparable DNA yields to the FastDNA SPIN Kit for Soil, which uses Lysing Matrix E tubes. The most suitable kit depends on sample characteristics and laboratory requirements.
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