MagBeads FastDNA® Kit for Blood
SKU: 116574096
MagBeads FastDNA® Kit for Blood is based on the purification method of high binding magnetic particles. The sample is lysed and digested. DNA is released into the lysate. After addition of magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove the proteins and impurities, washed with ethanol to remove salts, and finally the DNA was eluted with Elution Buffer.
Figure 1: Extraction Efficiency of MagBeads FastDNA Kit for Blood Using Manual Method and MPure™ System. The MagBeads FastDNA Kit for Blood showed high-quality and consistent gDNA extraction, with comparable purity and yield across both manual and MPure™ extraction methods
Figure 2: (A) gDNA was extracted from blood samples under different conditions and analyzed on a 1% agarose gel. Lanes are labeled as follows: "Short" (~1106 bp), "Medium" (~1424 bp), "Long" (~5778 bp), and "NTC" (Negative Template Control, no visible band). (B) Long fragments of the human BRCA gene were amplified via Endpoint PCR. DNA extracted with the MagBeads FastDNA Kit (MP) successfully produced the long fragment gene product, while DNA extracted with the Competitor T kit failed to amplify the long fragment.
Figure 3: DNA Integrity Analysis of Blood gDNA Extracted with MP MagBeads FastDNA Kit for Blood. Genomic DNA was extracted from 200 µL blood using the MP MagBeads FastDNA Kit and compared to other competitor kits. The MP MagBeads kit yielded DNA with superior integrity and higher molecular weight, as assessed by the Agilent Technologies 4150 TapeStation Genomic DNA ScreenTape system.
Application Notes
MagBeads FastDNA Kit for Blood is intended for extraction and purification of genomic DNA from whole blood and other bodily fluids. Isolated DNA is ready for sensitive molecular biology applications, including PCR, qPCR, NGS, Southern Blotting, Arrays, DNA Methylation, and more.
Usage Statement
Research Use Only (RUO)
Key Applications
Genomics, Molecular Biology, PCR, qPCR, NGS, Shotgun sequencing
| SKU | 116574096 |
| Alternate Names | Magnetic Bead DNA Extraction Kit for Blood, High-throughput DNA Extraction Kit for Blood, 96-well DNA Extraction Kit for Blood |
| Application Notes | MagBeads FastDNA Kit for Blood is intended for extraction and purification of genomic DNA from whole blood and other bodily fluids. Isolated DNA is ready for sensitive molecular biology applications, including PCR, qPCR, NGS, Southern Blotting, Arrays, DNA Methylation, and more. |
| Base Catalog Number | 6574096 |
| Product Line | MagBeads |
| Components | 3.5 mL Magnetic Beads, 50 mg Proteinase K, 3 mL Protease Dissolve Buffer, 60 mL Buffer MLA, 120 mL Buffer DW1, 60 mL Buffer CW, 60 mL Buffer BW3, 30 mL Elution Buffer |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Face shield; Gloves |
| Sample Type | Plasma, Serum, Blood, Bodily Fluid |
| Storage | Store at Room Temperature (15-30 °C) in a dry environment. Store Magnetic Beads at 2-8 ℃ upon arrival; do not freeze. |
| Usage Statement | Research Use Only (RUO) |
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