3,3-Diaminobenzidine tetrahydrochloride
3,3-Diaminobenzidine Tetrahydrochloride
BCIP/NBT, liquid substrate plus, 100 mL
BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.
3,3-Diaminobenzidine Tetrahydrochloride Tablets, 100 tabs
Used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry.
TMB (3,3',5,5' Tetramethylbenzidine)
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.