SPINeasy cfDNA Kit for Plasma and Serum

The SPINeasy cfDNA Kit for Plasma and Serum is a high performance kit designed for the simple yet efficient extraction of circulating cell-free DNA (cfDNA) from liquid biopsy samples, including plasma, serum, and urine. Leveraging advanced silica-membrane spin-column technology, this kit allows for rapid and reliable cfDNA isolation in under 60 minutes, without the need for homogenization. The specially formulated Buffer CF eliminates the need for homogenization and ensures efficient sample lysis within 30 min. The combination of its unique spin column (Column CF) and proprietary buffers are optimized to deliver concentrated, contaminant-free cfDNA that is ideal for a wide range of downstream applications, such as PCR, qPCR, and next-generation sequencing. This kit is an essential tool for researchers seeking high-quality cfDNA for liquid biopsy analysis.

 

 

Figure 1: Comparison of extraction efficiency of the SPINeasy cfDNA Kit for Plasma and Serum versus other competitor kits using plasma and urine samples. The SPINeasy kit demonstrates superior recovery of circulating free DNA (cfDNA) as analyzed by Agilent tapestation, highlighting its effectiveness for cfDNA isolation from both plasma and urine matrices.

Figure 2: qPCR analysis of short and long cfDNA fragments isolated using the SPINeasy cfDNA Kit for Plasma and Serum compared to other competitor kits. The SPINeasy® kit yields lower Ct values for short fragments (LINE66F), indicating higher recovery of smaller cfDNA fragments, while showing slightly higher Ct values for long fragments relative to a few other kits. 

Application Notes

The SPINeasy cfDNA Kit for Plasma and Serum is intended for purification of cell-free DNA from plasma and serum samples.

Usage Statement

Research Use Only (RUO)

Key Applications

PCR, real time PCR, and sequencing