Phenazine Methosulfate
Application Notes
Phenazine Methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used in tyrosine transaminase test
| SKU | 02100955-CF |
| Alternate Names | 5-Methylphenazinium methyl sulfate; N-Methylphenazonium methyl sulfate; PMS |
| Application Notes | Phenazine Methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported. |
| Base Catalog Number | 100955 |
| Beilstein Registry Number | 3898869 |
| Biochemical Physiological Actions | PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulfhydryl groups including those of enzymes. |
| CAS # | 299-11-6 |
| EC Number | 206-072-1 |
| Format | Powder |
| Hazard Statements | H315-H319-H335 |
| Molecular Formula | C14H14N2O4S |
| Molecular Weight | 306.336 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
| RTECS Number | SG1645000 |
| Safety Symbol | GHS07 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λ max (water) 384 - 388 nm |
![MP COA [100955] lot ID: M4057](https://www.mpbio.com/static/version1745479238/frontend/Mpbio/default/en_US/images/document.png "MP COA [100955] lot ID: M4057")
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