Product Description
Lysozyme
Application Notes
Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Germinative agent | Extracting group specific antigen
Specifications
| SKU | 0210083410 |
| Alternate Names | Muramidase; Mucopeptide N-acetylmuramoylhydrolase |
| Application Notes | Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts. |
| Base Catalog Number | 10083410 |
| Biochemical Physiological Actions | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. |
| CAS # | 9001-63-2 |
| EC Number | 235-747-3 |
| Extinction Coefficient | 26.4 (Lit.) |
| Format | Crystalline powder |
| Hazard Statements | H317 |
| Isoelectric Point | 11.0(Lit.) |
| Molecular Weight | 2235.597 g/mol |
| Pack Size | 10 g |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| RTECS Number | OL5987000 |
| Source | Chicken eggwhite |
| Specific Activity | ≥9000 Sugar U/mg solid |
| Unit Definition | One unit will cause a decrease in OD450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
![MP COA [100831] lot ID: M4808](https://www.mpbio.com/static/version1742393451/frontend/Mpbio/default/en_US/images/document.png "MP COA [100831] lot ID: M4808")
Datasheet
Datasheet