Pepsin, ≥1:10,000

Name: Pepsin, ≥1:10,000
SKU: 02102598-CF
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SKU: 02102598-CF

Key features and details

Acid protease used to digest proteins
Commonly used in the preparation of Fab fragments from antibodies
Contains the "cathepsin" component which has milk curdling activity
Broad range of substrate activity and demonstrates an esterase activity

Synonyms

Pepsin A

CAS Number:

9001-75-6

EC Number:

232-629-3

MDL Number:

pH 1.0

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Product Description

Pepsin

Application Notes

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids

Specifications

SKU	02102598-CF
Alternate Names	Pepsin A
Application Notes	Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.
Base Catalog Number	102598
Biochemical Physiological Actions	Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
CAS #	9001-75-6
EC Number	232-629-3
Extinction Coefficient	E1%280 = 14.7
Format	Powder
Hazard Statements	H315-H319-H334-H335
Personal Protective Equipment	Dust Mask, Eyeshields, Faceshields, Gloves
pKa	2.5-4.5 (2% aq soln)
Protein or Enzyme Type	Proteins, Enzymes & Peptides
RTECS Number	SC6132000
Solubility	Freely soluble in water; dissolves readily in 0.01 M HCl (0.5 mg/mL - clear, colorless solution); practically insoluble in alcohol, chloroform or ether.
Source	Porcine
Specific Activity	≥1:10,000
Storage	21
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.