P-Nitro blue tetrazolium chloride, molecular biology reagent

Name: P-Nitro blue tetrazolium chloride, molecular biology reagent
SKU: 02193999-CF
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SKU: 02193999-CF

Key features and details

P-Nitro Blue Tetrazolium Chloride

Synonyms

2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT

CAS Number:

298-83-9

Molecular Formula:

C40H30N10O6 • 2Cl

Molecular Weight:

817.644 g/mol

Beilstein Registry Number:

4115923

EC Number:

206-067-4

MDL Number:

MFCD00012159

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Product Description

P-Nitro Blue Tetrazolium Chloride

Application Notes

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Colorimetric detection of alkaline phosphatase conjugates

Specifications

SKU	02193999-CF
Alternate Names	2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
Application Notes	p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Base Catalog Number	193999
Beilstein Registry Number	4115923
CAS #	298-83-9
EC Number	206-067-4
Extinction Coefficient	260 nm: E1% = 740 (in water) (Lit.)
Format	Powder
Grade	Molecular Biology Grade
Hazard Statements	H302
Molecular Formula	C40H30N10O6 • 2Cl
Molecular Weight	817.644 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
RTECS Number	XF8045000
Safety Symbol	GHS07
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	255 ± 3 nm ( λ max (methanol))