Mouse anti-epithelial keratin-AE5 monoclonal antibody, 200 µg

Name: Mouse anti-epithelial keratin-AE5 monoclonal antibody, 200 µg
SKU: 08691431

In stock

SKU: 08691431

Product Description

Monoclonal Mouse Anti-Epithelial Keratin antibody is specific for the basic 64K keratin which, together with the acidic 55K keratin, has been designated as a marker for "corneal-type differentiation".

Application Notes

Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunoblot | Immunofluorescence assays (IFA) | Immunohistochemistry (Paraffin)

Specifications

SKU	08691431
Alternate Names	Type II Cytokeratin
Antibody Type	Monoclonal Antibody
Application Notes	Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.
Base Catalog Number	691431
Clone Name	AE5
Concentration	Antibody Conc.:1.0 mg/ml
Conjugate	Unconjugated
Format	lyophilized
Formulation Details	Liquid
Grade	Molecular Grade
Host	Mouse
Isotype	Purified Ig fraction from ascites
Pack Size	200 µg
Preparation Method	The AE5 monoclonal anti-keratin was prepared against human epidermal keratin by hybridoma technique using P3 x 63 Ag8 myeloma cells. The AE5 was reactive with the 64K keratins in the cornea.
Solubility	Soluble in water
Typical Working Concentration	The dilutions below are suggested as staring points. The dilution range for western blotting may be as great as 1:1000 - 1:3000. 1:500 (Immunoblot - human epithelial cornea) 1:50 (Immunofluorescence - human epithelial cornea) It is recommended that each lab obtain their own optimum working dilution.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.