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Spectinomycin, dihydrochloride pentahydrate, PhytoPure™

Spectinomycin, dihydrochloride pentahydrate, PhytoPure™

Synonyms U18409AE; Actinospectacin; M141; Trobicin

CAS Number: 22189-32-8 Molecular Formula: C14H24N2O7-2HCl-5H2O Molecular Weight: 495.40

EC Number: 244-554-3 MDL Number: MFCD00150886

SKU 02194540-CF
$39.20
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Description

Product Description

An aminocyclitol antibiotic, closely related to the aminoglycosides, it inhibits protein synthesis (elongation) by interfering with peptidyl tRNA translocation. It is active against gram-negative bacteria and is used as a selection agent for transformed plant cells that contain the selectable marker gene Spcr.

Application Notes

Specinomycin is used in cell culture applications, It is active against gram-negative bacteria.It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibiotic

Specifications
SKU 02194540-CF
Alternate Names U18409AE; Actinospectacin; M141; Trobicin
Application Notes Specinomycin is used in cell culture applications, It is active against gram-negative bacteria.It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons.
Base Catalog Number 194540
Biochemical Physiological Actions Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3'-adenyltransferase gene (aadA).Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
CAS # 22189-32-8
EC Number 244-554-3
Format Powder
Grade Cell Culture Grade
Molecular Formula C14H24N2O7-2HCl-5H2O
Molecular Weight 495.40
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number WG7400000
Source Streptomyces species
Specific Activity 500 µg/mg
Typical Working Concentration recommended for use in cell culture applications at 7.5-20 mg/L
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.