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Proteinase K Lyophilized Powder, ≥ 30 U/mg, Recombinant (Yeast)

Proteinase K Lyophilized Powder, ≥ 30 U/mg, Recombinant (Yeast)

$195.00
Recombinant Proteinase K is derived from yeast, ≥ 30 U/mg, lyophilized powder.

SKU: 02183990-CF

Molecular Weight:
29 kDa
EC Number:
3.4.21.64
Product Description

Proteinase K, a broad-spectrum serine protease, is first extracted and purified from Engyodontium album. It is named Proteinase K because it can degrade Keratin. The crystallization and molecular structure of Proteinase K indicates it belongs to subtilisin family, whose active site has a characteristic catalytic triple amino acid Asp39-His69-Ser224. Proteinase K has no significant substrate specificity and main cleavage site is the peptide bond at the carboxyl end of the hydrophobic amino acid such as aliphatic or aromatic. Recombinant Proteinase K is derived from yeast based on the optimized gene of Engyodontium album by site-directed mutation. It is purified by chromatography process and with calcium and glycerin as protective agents.

Application Notes

Extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

PCR, RNA Purification, DNA Purification, NGS, Sample Preparation

Specifications
SKU 02183990-CF
Application Notes Extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
Base Catalog Number 183990
EC Number 3.4.21.64
Grade Molecular Biology
Hazard Statements Non-hazardous
Molecular Weight 29 kDa
Preparation Method It is recommended to reconstitute the proteinase K powder with water, add 50% glycerin, or reconstitute with 50% glycerin. Store the reconstituted solution at 2-8 °C for long-term storage.
Purity >95%
Source Yeast
Specific Activity > 40 U/mg protein
Typical Working Concentration Working concentrations of the enzyme range from 50 to 250 µg/mL.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.