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Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It has been used in pre-extraction of membranes to remove peripheral proteins. It has been used as a blocking agent on nitrocellulose.
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Non-ionic detergent | Emulsifying agent | Blocking agent
|Alternate Names||Polysorbate 20; Polyoxyethylene (20) Sorbitan Monolaurate; Glycosperse L-20X; Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurate; TWEEN® 20; PEG(20)sorbitan monolaurate|
|Application Notes||Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. It has been used in pre-extraction of membranes to remove peripheral proteins. It has been used as a blocking agent on nitrocellulose.|
|Base Catalog Number||103168|
|Critical Micelle Concentration||0.06 mM|
|Molecular Weight||522.676 g/mol|
|Personal Protective Equipment||Eyeshields, Gloves|
|Refractive Index||n20/D 1.468(Lit.)|
|Solubility||Soluble in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>, <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a> and <a class="pubchem-internal-link CID-31275" href="https://pubchem.ncbi.nlm.nih.gov/compound/dioxane">dioxane</a>. Insoluble in mineral oil and petroleum ether|
|Specific Gravity||1.08 - 1.13|
|Sterilization of Solutions||Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.|
|Typical Working Concentration||As a blocking agent on nitrocellulose at a typical concentration of 0.05%. Used at concentration of 2% for extraction of membrane-bound proteins.|