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Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.
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Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids
|Alternate Names||Pepsin A|
|Application Notes||Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.|
|Base Catalog Number||195367|
|Biochemical Physiological Actions||Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.|
|Extinction Coefficient||E1%280 = 14.7|
|Isoelectric Point||pH 1.0|
|Personal Protective Equipment||dust mask, Eyeshields, Faceshields, Gloves|
|Protein or Enzyme Type||Proteins, Enzymes & Peptides|
|Safety Symbol||GHS07, GHS08|
|Source||Porcine Stomach Mucosa|
|Specific Activity||>2,000 u/mg protein|
|Unit Definition||One unit will produce a delta A280 of 0.001 per minute at pH 2.0 and 37°C, measured as TCA-soluble products using hemoglobin as substrate|