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Penicillin G, potassium salt, cell culture reagent
Penicillin G Potassium Salt
Penicillin G potassium salt is used as a cell culture additive as an antibiotics.
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Cell culture reagent | Antibiotic | Inhibitor of bacterial cell wall synthesis
|Alternate Names||Benzylpenicillin Potassium Salt|
|Application Notes||Penicillin G potassium salt is used as a cell culture additive as an antibiotics.|
|Base Catalog Number||194536|
|Beilstein Registry Number||3832841|
|Biochemical Physiological Actions||Mode of Action: Use to inhibit the synthesis of bacterial cell walls by inhibition of the cell wall peptidoglycan chain cross-lining. Antimicrobial spectrum: Gram-positive bacteria.|
|Density||1.41 (NTP, 1992)|
|Grade||Cell Culture Grade|
|Melting Point||408 to 414° F (decomposes) (NTP, 1992)|
|Molecular Weight||356.372 g/mol|
|Personal Protective Equipment||Dust mask, Eyeshields, Faceshields, Gloves|
|Potency||1,500 - 1,700 u/mg|
|Preparation Method||To prepare Stock Solutions: Add the appropriate amount of powder to water while mixing (not the reverse), as adding water to the powders may result in insoluble mixtures that cannot be filtered without losing potency. If desired, the pH may be adjusted, but a basal media with greater than 2 g/L sodium bicarbonate can easily absorb up to 2% of an acidic antibiotic solution; therefore, an antibiotic solution with an acidic pH would not influence the pH of the media. After ensuring that the resultant solution is transparent, sterile filter and store in small aliquots at appropriate temperature until use. Prior to use of the media, aseptically add the appropriate amount of antibiotic to the basal media in order to obtain the required working concentration. Mix well and sterile filter if asepsis is believed to have been compromised.|
|Solubility||50 to 100 mg/mL at 77° F (NTP, 1992)|
|Typical Working Concentration||Gram Positive Bacteria - 105 U/L|