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O-Nitrophenyl-β-D -galactoside, 5 g

O-Nitrophenyl-β-D -galactoside, 5 g

Synonyms o-Nitrophenyl-β-D -galactoside; 2-Nitrophenyl -D-galactopyranoside; ONPG; 2-Nitrophenyl β-D-galactopyranoside

CAS Number: 369-07-3 Molecular Formula: C12H15NO8 Molecular Weight: 301.3

Beilstein Registry Number: 92207 EC Number: 206-716-1 MDL Number: MFCD00063255

Packaging size: 5 g

SKU 0219403005
$123.15
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Description

Product Description

o-Nitrophenyl β-D-galactopyranoside is a colorimetric substrate for β-galactosidase.

Application Notes

o-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase. It is also used as a cell culture component for molecular genetics.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell culture component for molecular genetics | Substrate for β-galactosidase

Specifications
SKU 0219403005
Alternate Names o-Nitrophenyl-β-D -galactoside; 2-Nitrophenyl -D-galactopyranoside; ONPG; 2-Nitrophenyl β-D-galactopyranoside
Application Notes o-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase. It is also used as a cell culture component for molecular genetics.
Base Catalog Number 19403005
Beilstein Registry Number 92207
Biochemical Physiological Actions β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
CAS # 369-07-3
EC Number 206-716-1
Melting Point 190 - 202°C
Molecular Formula C12H15NO8
Molecular Weight 301.3
Pack Size 5 g
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance UV/VIS: λ max (water): 262 ± 3 nm