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Melatonin ELISA kit, 1 kit

Melatonin ELISA kit, 1 kit

Packaging size: 1 kit

SKU 0219359601
$1,311.95
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Specifications
SKU 0219359601
Application Notes The Melatonin ELISA is a competitive enzyme immunoassay for the quantitative determination of melatonin in serum without extraction. The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and non-biotinylated antigen for a fixed number of antibody binding sites.
Base Catalog Number 19359601
Biochemical Physiological Actions The Melatonin ELISA is a competitive enzyme immunoassay for the quantitative determination of melatonin in serum. The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
Pack Size 1 kit
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.