GeneClean® Turbo Kit is a flexible kit designed for purification of DNA fragments of sizes 0.1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. It purifies larger fragments due to less manipulation of bound DNA. Process with either vacuum or centrifugation & save time with patented Turbo filter design.
GENECLEAN® Turbo Kit is used for purification of DNA fragments of sizes 0.1 kb to 300 kb from agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Purification of DNA fragments from agarose gels; PCR reactions | Elimination of proteins from enzymatic reactions
|Alternate Names||DNA Purification|
|Application Notes||GENECLEAN® Turbo Kit is used for purification of DNA fragments of sizes 0.1 kb to 300 kb from agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.|
|Base Catalog Number||1102200|
|CAS #||GUANIDINE THIOCYANATE 593-84-0; WATER 7732-18-5|
|Components||GENECLEAN® Turbo Salt Solution(14 mL) |GENECLEAN® Turbo Wash Concentrate(1.5 mL) |GENECLEAN® Turbo Cartridges(10 cartridges) |GENECLEAN® Turbo Catch Tubes(10 tubes) |GENECLEAN® Turbo Elution Solution(1 mL) |GENECLEAN® Turbo GNomic Salt Solution(14 mL)|
|Format||GLASSMILK™ imbedded GENECLEAN® Turbo SPIN column|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Preparation Method||Before first use, add the 13.5 mL of 200 proof (100% ethanol) to the GENECLEAN® Turbo Wash Concentrate (1.5 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.|
|Sample Source||Gels and Solutions|
|Sample Type||Enzymatic Reactions, Solutions, Agarose Gels|
|Sample Volume||Up to ~100 mg gel slice or up to ~100 µl DNA solution|
|Unit Definition||Each GENECLEAN® Turbo Cartridge has a binding capacity of 10 μg of either ssDNA or dsDNA.|