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The GENECLEAN® III kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.
The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA
|Alternate Names||DNA Clean-Up Kit|
|Application Notes||The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.|
|Base Catalog Number||1001600|
|Components||NaI| New wash concentrate| TBE modifier| EZ-Glassmilk™| Label Block| Elution Solution| BioFloat™ floating incubation rack|
|Pack Size||600 Preps|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Above 500 bp: 4.5 M NaI pH 7.0 - 7.4| Between 200 - 500 bp: 4.5 M NaI pH 6.0| NEW Wash: pH Range: 7.4 - 8.0| TBE Modifier: pH 5.8 ± 0.2|
|Preparation Method||Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.|
|Recommended Alternate||GENECLEAN® SPIN Kit|
|Safety Symbol||GHS05, GHS06|
|Sample Volume||Up to ~0.5 ml gel slice by volume or ~1.0 ml solution|
|Unit Definition||One µL of EZ-GLASSMILK™ will bind with one µg of DNA in 4.5 M Nal in 5 minutes at room temperature.|