The FastProtein Red Matrix is optimized for the rapid lysis of algae, yeast and fungi. Cells are resuspended in either the provided Yeast Breaking Buffer (YBB) or other expression buffer, then added to the Lysing Matrix C tube (1mm silica beads). After 20-40 seconds processing in a FastPrep® Instrument or equivalent bead-beater, proteins can be extracted from the centrifuged homogenate using a wide variety of methods.
Designed for rapid isolation and purification of protein from yeast, algea and fungi cultures. The process is designed to be a small scale test for protein induction.
Protein isolation from yeast, fungi and algae.
|Application Notes||Designed for rapid isolation and purification of protein from yeast, algea and fungi cultures. The process is designed to be a small scale test for protein induction.|
|Base Catalog Number||6550600,6550700|
|Components||Contains 2.0 mL tubes of Lysing Matrix C containing 1.0mm silica spheres, 250 mL of protein isolation buffer and protocol for protein isolation from yeast and fungi.|
|FastPrep Instruments Models||ConeFlex™ Legacy (allows all FastPrep-24™ adapters to be used on the FastPrep-96™.), CoolPrep™ (24x2 mL), HiPrep™ (48x2 mL), QuickFlex™ (96x2 mL), QuickPrep™ (24x2 mL)|
|Personal Protective Equipment||If working with biological, toxic, hazardous or infectious samples use hand protection (latex, nitrile or other plastic material gloves), skin and body protection (labcoat and sleeves) and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practice.|
|Sample Volume||up to 200 mg per prep|