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Tricine, electrophoresis grade, ≥99%

Tricine, electrophoresis grade, ≥99%

$60.90

Key features and details

Tricine for use in electrophoresis buffers

SKU: 04807410-CF

Synonyms
N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
CAS Number:
5704-04-1
Molecular Formula:
C6H13NO5
Beilstein Registry Number:
1937804
EC Number:
227-193-6
Product Description

Tricine

Application Notes

Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Specifications
SKU 04807410-CF
Alternate Names N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
Application Notes Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Base Catalog Number 807410
Beilstein Registry Number 1937804
CAS # 5704-04-1
EC Number 227-193-6
Format Powder
Grade Electrophoresis grade
Hazard Statements H317
Insoluble Matter ≤10 ppm
Molecular Formula C6H13NO5
Optical Rotation DpK/DT: -0.021(Lit.); Metal Binding Constants (log K) for 0.1 M solution at 20 °C: Mg2+: 1.2 Ca2+: 2.4 Mn2+: 2.7 Cu2+: 7.3(Lit.)
pKa 4.5 - 5.5 (1% aq soln) ; Buffering pH range 7.4-8.8(Lit.)
Potency 8.1 (25 °C)(Lit.)
Purity ≥99%
Source Very soluble in water (25% w/v - clear; colorless solution).
Storage 24
Unit Definition A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis (COA)