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Chloroform

Chloroform

$37.85

Key features and details

  • Molecular Biology Reagent
  • ≥99%

SKU: 02194002-CF

Synonyms
1,1,1-Trichloromethane; Trichloromethane; Methylidyne trichloride; Triclorometano; Formyl trichloride
CAS Number:
67-66-3
Molecular Formula:
CHCl3
Molecular Weight:
119.369 g/mol
Beilstein Registry Number:
1731042
EC Number:
200-663-8
MDL Number:
MFCD00000826
Product Description

Chloroform

Application Notes

Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Solvent | Cleansing agent

Specifications
SKU 02194002-CF
Alternate Names 1,1,1-Trichloromethane; Trichloromethane; Methylidyne trichloride; Triclorometano; Formyl trichloride
Application Notes Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Auto Ignition Not flammable (USCG, 1999)
Base Catalog Number 194002
Beilstein Registry Number 1731042
Boiling Point 143° F at 760 mm Hg (EPA, 1998)
CAS # 67-66-3
Density 1.4832 at 68° F (EPA, 1998)
EC Number 200-663-8
Flash Point None (EPA, 1998)
Grade Molecular Biology Grade
Hazard Statements H302-H315-H351-H373
Melting Point -82.3° F (EPA, 1998)
Molecular Formula CHCl3
Molecular Weight 119.369 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge
Purity ≥99%
Refractive Index n20/D 1.445(Lit.)
RTECS Number FS9100000
Safety Symbol GHS07, GHS08
Solubility less than 1 mg/mL at 66° F (NTP, 1992)
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Vapor Density 4.12 (EPA, 1998) (Relative to Air)
Vapor Pressure 100 mm Hg at 50.72° F (EPA, 1998)
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