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EMM is a minimal defined medium for the growth of S. pombe, containing dextrose, buffers, salts, vitamins and trace minerals. Dextrose is the energy source. EMM minimal defined medium is thiamine-free nmt1 promoter studies in Schizosaccharomyces pombe.
EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.
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Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
|Alternate Names||Edinburgh's Minimal Medium |S.pombe growth medium|
|Application Notes||EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.|
|Base Catalog Number||4110012|
|Formulation Details||Contents Per Liter: 3 g potassium hydrogen phthalate (14.7mM); 2.2g Na2HPO4 (15.5mM); 5g NH4Cl (93.5mM); 20g glucose (111mM); 1.05g MgCl2 - 6H2O (5.2mM); 14.7µg CaCl2 - 2H2O (99.8µM); 1g KCl (13.4mM); 0.04g Na2SO4 (0.28mM); 1mg pantothenic acid (4.2µM); 10mg nicotinic acid (81.2µM); 10mg myo-inositol (55.5µM); 10µg biotin (0.82µM); 0.5 mg boric acid (8.9µM); 0.4mg MnSO4 (2.4µM); 0.4mg ZnSO4 - 7H2O (1.4µM); 0.2mg FeCl2 - 6H2O (0.7µM); 40µg molybdic acid (0.25µM); 0.1mg KI (0.6µM); 40µg CuSO4 - 5H2O (0.016µM); 1mg citric acid (4.76µM).|
|Grade||Molecular Biology Certified™|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range: 5.4 - 6.4 at 25 °C|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM medium formulations. Chosen reagents are combined in the proper proportions according to the EMM formula and milled to a powder of consistent sized particles. A sample of the milled formulation was used to prepare liquid and agar medium by adding 32.2 g powder per liter of purified water. For agar medium 17g/L of agar-Y was added. The combinations were mixed by hand for about 1 minute to dissolve the glucose and 0.25g/L of a mixture of adenine, his, leu, ura, and lys (Sp supplements, Cat.# 4104012) was added. Autoclaving is not recommended as it may cause precipitation. Sterile filter the solution into a sterile bottle.|
|Solubility||Soluble in water (32.2 g/L).|