DOB allows for preparation of a custom synthetic defined yeast media and includes a complete yeast nitrogen base (YNB) composed of buffering salts, vitamins and trace elements, with a carbon source.
DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.
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Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Dropout Base Medium |S.cerevisiae Growth medium|
|Application Notes||DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.|
|Base Catalog Number||4025012|
|Formulation Details||Contents/liter: 1.7 g YNB; 5g Ammonium Sulfate; 20 g Dextrose|
|Grade||Molecular Biology Certified™|
|Media Type||Yeast Dropout Base (DOB)|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||5.0 ± 0.2|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in DOB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution.|
|Solubility||Soluble in Water (26.7 g/L)|
|Typical Working Concentration||26.7g/liter|