Terrific Broth II is a proprietary reformulation of the classic Terrific Broth rich medium for high density growth of E. Coli cells and higher yields of plasmid DNA without the need of antibiotic amplification. Similar in functin to CIRCLEGROW™ Terrific Broth II is a single powder mixture that eliminates the 2 part preparation method of the original Terrific Broth formulation.
Terrific Broth II is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
|Alternate Names||TB medium|
|Application Notes||Terrific Broth II is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.|
|Base Catalog Number||3046022|
|Formulation Details||Contents Per Liter: Proprietary formulation containing Yeast Extract; Tryptone Broth; Sodium Chloride and Vitamins.|
|Grade||Molecular Biology Grade|
|Media Type||Terrific broth II|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.84±0.3 at 25 °C. Please note that the pH of Terrific Broth II Medium liquid increases as the temperature of the solution decreases.|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Terrific Broth II formulations. Chosen reagents are combined in the accurate proportions according to the formula and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and autoclaved at 121 °C for 15 minutes.|
|Solubility||Soluble in water (50 g/L).|