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50X Trypsin-EDTA solution, 100 mL

50X Trypsin-EDTA solution, 100 mL

$29.90

Key features and details

0.25% Trypsin (1:250) and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL0

SKU: 091689649

Synonyms
Trypsin (1X), Trypsin-EDTA solution
CAS Number:
9002-07-7 (trypsin)
Molecular Weight:
Approximately 23;000 (trypsin)
Packaging size:
100 mL
Specifications
SKU 091689649
Alternate Names Trypsin (1X), Trypsin-EDTA solution
Application Notes Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cellto-cell adhesion and obscure the peptide bonds on which trypsin acts.
Base Catalog Number 1689649
CAS # 9002-07-7 (trypsin)
Concentration 0.25% (w/v) solution | 0.25% Trypsin (1:250) and 1mM EDTA In Modified HBSS Without Ca++ and Mg++
Format Solution
Formulation Details Liquid | 0.25% Trypsin (1:250) and 1mM EDTA In Modified Hbss Without Ca++ and Mg++
Media Type Mammalian Media
Molecular Weight Approximately 23;000 (trypsin)
Pack Size 100 mL
pH 7.0 - 7.3
Source Porcine (for trypsin)
Specific Activity 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
Specificity Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with α2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Sterility Sterile
Unit Definition One BAEE unit will produce a ΔA253 of 0.001 per minute at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path). One TAME unit hydrolyzes 1 μmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25 °C, pH 8.2, in the presence of 0.001 M calcium ion. One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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Material Safety Datasheet
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