Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.
- Maximum yield of mononuclear cells
- Easy one-step centrifugation
- >96% cell viability of lymphocytes
- Low endotoxins
- Autoclave sterilization
Check out our latest article in Genetic Engineering & Biotechnology News about High-Performance Density Gradient Media for Purification of Leukocytes from Blood.
LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.
In vitro isolation of lymphocytes from diluted whole blood.
|Application Notes||LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.|
|Base Catalog Number||1692249,1692254|
|Formulation Details||Liquid - Lymphosep is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v).|
|Media Type||Lymphocyte Separation Media|
|pH||6.0 - 9.0|
Exposures to the environmental toxicants pentachlorophenol (PCP) and dichlorodiphenyltrichloroethane (DDT) modify secretion of interleukin 1-beta (IL-β) from human immune cells.