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Tricine

Tricine

$26.95

Key features and details

Tricine

SKU: 02103112-CF

Synonyms
N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
CAS Number:
1389475
Molecular Formula:
C6H13NO5
Beilstein Registry Number:
1937804
EC Number:
227-193-6
MDL Number:
MFCD00004277
Product Description

Tricine

Application Notes

Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. It is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Buffer | Cryopreservation medium | Scavenger of hydroxyl radicals

Specifications
SKU 02103112-CF
Alternate Names N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
Application Notes Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. It is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Base Catalog Number 103112
Beilstein Registry Number 1937804
CAS # 1389475
EC Number 227-193-6
Format Powder
Molecular Formula C6H13NO5
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pKa 8.1 at 25 °C(Lit.)
Sterilization of Solutions To sterilize solutions, MP recommends filter sterilization using a 0.2 micron filter.
Typical Working Concentration A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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